Plasmid Purification-Kits I-Blue Mini / Midi
For rapid and efficient extraction of plasmid DNA from bacterial cells.
The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.

-Fast extraction time
-High yield
-Prevention of handling errors during cell lysis and neutralisation by I-Blue colour indicator
Specifications I-Blue Mini Plasmid Kit // I-Blue Midi Plasmid Kit
Method: Spin column // Anion-Exchange
Sample size: 1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmid
Binding capacity: 50 µg // 500 µg
Fragment size: 1 - 15 kb // 1 - 20 kb
Typical yield: up to 50 µg // 200 - 500 µg
Operation Time: <15 min. // <80 min.

Type I-Blue MIDI
Description Gravity flow
For 25 preps